Biosynthesis of the Coat Protein of Coliphage F2 by Extracts of Euglena Gracilis.
نویسندگان
چکیده
(1963); Weinberg, C. J., D. R. Nelson, J. G. Carter, and L. G. Augenstein, J. Chem. Phys., 36, 2869 (1962); Weissbluth, M., personal communication. 4 Porter, G., Proc. Roy. Soc., (London), A200, 284 (1950). 5 Hastings, J. W., W. H. Riley, and J. Massa, J. Biol. Chem., in press. 6 Hastings, J. W., J. A. Spudich, and G. Malnic, J. Biol. Chem., 238, 3100 (1963). 7 Weber, G., in Light and Life, ed. McElroy and Glass (Baltimore: Johns Hopkins Press, 1961), p. 82. 8 Beinert, H., in The Enzymes, ed. Boyer, Lardy, and MyrbAck (New York: Academic Press, 1960), vol. 2, p. 339; Massey, V., in The Enzymes, ed. Boyer, Lardy, and Myrback (New York: Academic Press, 1963), vol. 7, p. 275. 9 Strehler, B. L., and W. A. Arnold, J. Gen. Physiol., 34, 809 (1951). 10 Nickerson, W. J., and G. Strauss, J. Am. Chem. Soc., 83, 3187 (1961). 1' Sistrom, W. R., and R. K. Clayton, Biochim. Biophys. Acta, 88, 61 (1964). 12 Clayton, R. K., personal communication.
منابع مشابه
Different Porphobilinogenases in Cytoplasm and Isolated Chloroplasts from Light-Grown Euglena gracilis Z
Fachbereich Biologie/Botanik, Philipps-Universität Marburg. D-3550 Marburg. Bundesrepublik Deutschland Z. Naturforsch. 44c, 81 — 84 (1989); received September 26. 1988 Porphyrinogens. Euglena gracilis Z ., Porphyrin Biosynthesis. Porphobilinogenase. Euglena Chloroplasts Lysed cells as well as different fractions of isolated, disrupted chloroplasts from light grown Euglena gracilis were tested f...
متن کاملPurification, characterization, and immunological properties of fumarase from Euglena gracilis var. bacillaris.
A rapid three-step procedure utilizing heat treatment, ammonium sulfate fractionation, and affinity chromatography on Matrex gel Orange A purified fumarase (EC 4.2.1.2) 632-fold with an 18% yield from crude extracts of Euglena gracilis var. bacillaris. The apparent molecular weight of the native enzyme was 120,000 as determined by gel filtration on Sephacryl S-300. The preparation was over 95% ...
متن کاملTryptophan synthetase in Euglena gracilis strain G.
The five enzyme activities in the synthesis of l-tryptophan have been obtained in extracts of Euglena gracilis. One of these, tryptophan synthetase, has been studied in detail. The general catalytic properties of tryptophan synthetase, including the range of reactions catalyzed and its substrate and cofactor affinities, are similar to those reported for other organisms. The Euglena enzyme has t...
متن کاملEnergy-Saving Lipid Extraction from Wet Euglena gracilis by the Low-Boiling-Point Solvent Dimethyl Ether
We tested a wet extraction method for lipid extraction from Euglena gracilis water slurry at 0.51 MPa and 20 °C using liquefied dimethyl ether (DME). The yields, proximate analyses, elemental composition, and molecular weight distribution properties of the extracts from E. gracilis and the remaining residues obtained by DME extraction were compared with those of the extracts obtained by hexane ...
متن کاملPreparation of chloroplasts from euglena highly active in protein synthesis.
Chloroplasts can be obtained by gentle lysis or mild shear of spheroplasts of vitamin B(12)-deficient Euglena gracilis and then purified by isopycnic sedimentation on gradients of Ludox AM or Percoll. The chloroplasts appear compact and highly refractile by phase contrast or Hoffmann contrast microscopy. Upon incubation with [(3)H]leucine or [(35)S]methionine, the chloroplasts incorporate the a...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Proceedings of the National Academy of Sciences of the United States of America
دوره 53 شماره
صفحات -
تاریخ انتشار 1965